贺武汉大学化学与分子科学学院应用CHI Scientific产品成功发表SCI论文(IF:5.748)
HUVECs(7-1012) , complete endothelial cell growth medium ;CHI Scientific
An artificial blood vessel implanted three-dimensional microsystem for modeling transvascular migration of tumor cells
Lab Chip, 2015, 15, 1178–1187
Xue-Ying Wang1, Ying Pei1, Min Xie1, Zi-He Jin1, Ya-Shi Xiao1, Yang Wang1, Li-Na Zhang1, Yan Li1 and Wei-Hua Huang1
1 Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular
Sciences, Wuhan University,
1 College of Chemistry and Molecular Sciences, Wuhan University, Wuhan,
1 Department of Oncology, Zhongnan Hospital of Wuhan University, Wuhan,
Abstract
Here, we report an artificial blood vessel implanted 3D microfluidic system for reproducing transvascular migration of tumor cells.
The transparent, porous and elastic artificial blood vessels are obtained by constructing polysaccharide cellulose-based microtubes
using a chitosan sacrificial template, and possess excellent cytocompatibility, permeability, and mechanical characteristics.
Methods
This model consists of a peristaltic pump to manipulate fluids and a cellulose/collagen artificial blood vessel implanted collagen chip.
The human umbilical vein endothelial cells (HUVECs) (CHI Scientific, Inc., USA) were cultured using complete endothelial
cell growth medium (CHI Scientific, Inc., USA) in the lining of the cellulose/collagen tubes.
Results
The results demonstrated that this in vitro model using a natural polysaccharide scaffold with collagen stuffing could well mimic the
structure and function of the vascular wall of microvessels with 3D endothelial monolayers.
Conclusions
In this work, we develop an artificial blood vessel implanted 3D microsystem for modeling transvascular migration of tumor cells. The
transparent, elastic and porous cellulosebased microtubes with 3D endothelium well mimic the structure and functions of the blood
vessel.